|
KMID : 1190620050020010014
|
|
Journal of the Korean Wound Care Society
2005 Volume.2 No. 1 p.14 ~ p.18
|
|
|
Comparison of Effect of Autogenic, Allogenic, Xenogenic Serum Supplementations in Fibroblast Culture
|
|
Lee Min-Ah
Han Seung-Kyu
Kim Jeong-Bae
Kim Woo-Kyung
|
|
|
Abstract
|
|
|
|
Expanding cells ex-vivo is important in tissue-engineering. Culture medium is usually supplemented with fetal bovine serum (FBS) in most of experiments. However, cells grown in bovine serum media have possibilities of disseminating bovine diseases and/or causing patient’s immune reaction. To overcome these problems, autologous or homologous serum should be used instead of FBS. The purpose of this study is to compare cell proliferation and collagen synthesis depending on the kind of sera mixed on media and to provide the guideline on applying established experimental data to clinical cases. Human dermal fibroblasts were obtained from four patients. Five thousand cells per well in 96-well plates were incubated DMEM/F-12 Nutrient with varying serum mixture; 10% autologous serum, 10% homologous serum, and 10% FBS. Five days after incubation fibroblast proliferation and collagen production were determined by MTT assay and CICP enzyme immunoassay. The means of cell number were 3.95?104/well, 2.97?104/well, and 2.30?104/well, respectively. The average amounts of collagen synthesized were 238.13?15.6 ng/ml, 204.88?13.5 ng/ml, 163.88?9.9 ng/ml in each. These results show that use of human serum mixture may contribute to not only exclude infection of bovine diseases and/or the patient’s immune reaction, but also increase cell proliferation and collagen synthesis. (J Korean Wound Care Soc 2006;2:14-18)
|
|
|
KEYWORD
|
|
|
Serum, Culture medium, Fibroblast, Collagen
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|